P/1 DNA

In this study, we only utilize one kind of probe-1 (P-1) DNA-conjugated polymer and easily set probe-2 (P-2) DNA on substrate, through hybridization between P-1 DNA and P-2 (Fig. 11) to analyze various target DNA, resulting in a DNA sensor that can analyze two different types of target DNA and distinguish fully matched from single nucleotide polymorphism (SNP) sequences. 

Using PAA as the starting point, a self-assembly DNA-conjugated polymer was prepared for DNA chip fabrication. The amounts of ssDNA and PDPH in the polymer were determined by absorption measurements to be equivalent to 1/714 [molecule/monomerunit] and 1/46 [molecule/monomerunit], respectively. A 20-mer ssDNA as P-1 DNA and PDPH for self-assembled immobilization were covalently attached to PAA as side chains. After self-assembled immobilization of the DNA-conjugated polymer on the gold surface of a sensor, the P-1 DNA chain was hybridized to a 34-mer ssDNA as P-2 DNA, which had a sequence fully matched to the desired target DNA. Analysis of the first hybridization (between P-1 and P-2 DNA) and of the second hybridization (between P-2 and the target DNA) was done by fluorescence measurements. 

As a control experiment, the fully matched DNA (i.e., fully matched to P-2) was dropped onto immobilized P-1 DNA-conjugated polymer lacking P-2 DNA at 0.3 M NaCl. Its relative fluorescence intensity was 8.8%, indicating that no hybridization occurred. These results indicated that observed binding was due to selective hybridization between P-2 DNA and the fully matched sequence on the gold surface. 

Fig. 6.5(a) OR gene expression. M, maternal and P, paternal DNA strands 1 2, chromosomes from neurones with differential cis/trans regulation (M/P) of each DNA strand (from Chess et al., 1998). [ = OR gene locus = suppressed regulatory element]... 

Sickle cell disease is caused by a mutation that results in the substitution of a valine residue for a glutamate residue in the sixth position of the hemoglobin P-chain. This results from the substitution of a T for an A in the glutamate codon. When (1) DNA from a patient... 

Carell has recently presented the study of a flavin amino acid chimera to model riboflavin in DNA photolyases [68]. This amino acid LI (Fig. 20) was synthesized in an enantiopure fashion by building the alloxazine ring onto the epsilon amine of lysine. This coenzyme chimera was applied to the problem of repairing DNA damage caused by UV irradiation. LI was incorporated into an 21-residue peptide, P-1, possessing the sequence of the DNA-binding domain of the helix-loop-helix transcription factor MyoD. 

Inject an appropriate volume of DNA solution (2-200 p.1) onto the column. 

Prepare a purification gel by adding an equal amount of forma-mide loading buffer to the resuspended DNA pool and a small amount of P-labeled DNA from step 1. 

Blair, D. and McManus, D.P. (1 989) Restriction enzyme mapping of ribosomal DNA can distinguish between fasciolid (liver fluke) species. Molecular and Biochemical Parasitology 36, 201-208. 

The initial event in the nucleotide incorporation cycle is binding of the double-stranded p/t DNA to the polymerase to form the E p/t complex (Step 1 Fig. 1). Many structures now exist, in which DNA is bound at the polymerase active site. 

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