P400 protein

Cloning of the P400 protein cDNA revealed that it was identical to the IP3 receptor protein, as well as the Purkinje cell-specific PCPP-260 protein isolated by Walaas et al. 

Fig. 23. Section of reeler mutant mouse cerebellum stained with monoclonal antibody 4C11 against the P400 protein. Note stained Purkinje cells in the cortex (CX) and in the central mass of dislocated cells (DP). Bar = 200 jum. Maeda et al. (1989).

Fig. 24. Sagittal sections of mouse cerebella of various ages stained with a monoclonal antibody (4C11) against the P400 protein.The samples were from (A) postnatal day O (PO), (B) P3, (C) P5, (D) P7, (E) PIO, (F) P15, and (G) P20 cerebellum. A section from a P20 old mouse cerebral cortex did not react with same antibody (H). Magnification lOOx. Maeda et al. (1989).

Several polypeptides, that are present in all Purkinje cells, but not in other cells of the cerebellum, have been mentioned in the previous sections of this chapter. They include the IP3 receptor (identical to the P400 protein and to the PCPP-260 protein of Walaas et al., 1986) (see Section 3.1.4), IPj-S-kinase (Section 3.1.4), cGMP-dependent protein kinase (Section 3.1.5), PEP-19 and calbindin-D28K (Section 3.1.7). Two other... 

Maeda N, Niinobe M, Nakahira K. Mikoshiba K (1988) Purification and characterization of P400 protein, a glycoprotein characteristic of Purkinje cell, from mouse cerebellum. J. Neurochem., 51, 1724-1730. 

Maeda N, Niinobe M, Inoue Y, Mikoshiba K (1989) Developmental expression and intracellular location of P400 protein characteristic of Purkinje cells in the mouse cerebellum. Dev. Biol., 133, 67-76. 

Mikoshiba K, Huchet M, Changeux JP (1979) Biochemical and immunological studies on the P400 protein, a protein characteristic of the Purkinje cell from mouse and rat cerebellum. Dev. Neuroscl, 2, 254-275. 

NuA4 Nucleosome acetyl transferase of histone H4. A multisubunit complex which includes, notably, TRRAP, p400 and Tip60 proteins. 

Furtrichi T, Yoshikawa S, Miyawaki A, Wada K, Maeda N, Mikoshiba K. Primary structure and functional expression of the inositol 1,4,5-trisphosphate-binding protein P400. Natrrre 1989 342 32-38. 

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