Oxygen binding, hemoglobin, calculations

Fig. 1.2. Fractions of molecules of hemoglobin occupied by a different number of oxygen molecules (m f.) as a function of the fraction of occupied oxygen binding sites in hemoglobin. Curves are calculated by using parameters of the Adair equation given in Fig. 1.1.

Calculate the fractional saturation YT of the tetramer of human hemoglobin with molecular oxygen using the equilibrium constants determined by Mills, Johnson, and Akers (1976) at 21.5 °C, 1 bar, pH 7.4, [Cl"] = 0.2 M and 0.2 M ionic strength. Make the calculation with the Adair equation and also by using the binding polymomial YT. 

One reason carbon monoxide (CO) is toxic is that it binds to the blood protein hemoglobin more strongly than oxygen does. The bond between hemoglobin and CO absorbs radiation of 1953 cm . (The units are the reciprocal of the wavelength in centimeters.) Calculate the wavelength (in nm and A) and the frequency (in Hz) of the absorbed radiation. 

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