2-Oxoglutarate carrier

The storage role of (Cu,Zn)-SOD in seeds e.g. seems plausible, when the Cu-carrier function of ceruloplasmin is considered The lipophilic anti-inflammatory and anti-ulcer Cu-chelates could also raise the Cu concentration in certain tissues and thus enhance their lysyl oxidase activity. But especially Cu(acetylsalicylate)2 inhibited protine,2-oxoglutarate dioxygenase (EC 1.14.11.2) and lysine,2-oxoglutarate dioxygenase (EC 1.14.1.4), which are also important enzymes in the processing of collagen... 

Assay techniques GDH utilizes both nicotinamide nucleotide cofactors NAD+ in the direction of N liberation (catabolic) and NADP+ for N incorporation (assimilatory). In the forward reaction, GDH catalyzes the synthesis of amino acids from free ammonium and Qt-kg. The reverse reaction links amino acid metabolism with TCA cycle activity. In the reverse reaction, GDH provides an oxidizable carbon source used for the production of energy as weU as a reduced electron carrier, NADH, and production of NH4+. As for other enzymes, spectrophotmetric methods have been developed for measuring oxoglutarate and aminotransferase activities by assaying substrates and products of the GDH catalyzed reaction (Ahmad and Hellebust, 1989). 

Composition of the reagent carrier (proportionate mass percentage to) 0.8% Potassium phosphate, monobasic 4.2% 2-Oxoglutarate 30.6% L-aspartate... 

Determination of ALT ALT (formerly glutamate pyruvate transaminase) catalyzes the equilibrium transfer reaction of the amino group from L-alanine to 2-oxoglutarate to form L-glutamate and pyruvate ALT requires pyridoxal phosphate as coenzyme, which acts as an amino carrier. It is found in the main organs, such as the liver, kidney, and heart. The ALT activity in serum is elevated in diseases of the liver. 

In the oxidative decarboxylation of pyruvate and oxoglutarate, as shown in Figure 3, TPP functions as a carrier of active aldehyde to form the intermediates, hydroxyethyl-TPP and a-hydroxy-P-carboxypropyl-TPP, which are finally transferred to coenzyme A (CoA) to form acetyl-CoA and succinyl-CoA, respectively. 

CO2 and the generation of reduced hydrogen carriers. A number of cycle intermediates may be used in biosynthetic pathways. Oxaloacetate and 2-oxoglutarate may be converted into aspartate and glutamate respectively by amino transfer reactions (Section 16.1) and thereby be employed as sources of these amino acids for protein synthesis. 

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