Oxidative cleavage, degradation with hydroxide

For the oxidative base lesions discussed in this section, the phosphoramidite of 8-oxo-dG is commercially available. For coupling on a DNA synthesizer, no changes are required from the standard method recommended by the synthesizer manufacturer. However, for deprotection and cleavage from the solid support with ammonium hydroxide, 0.25 M 2-mercaptoethanol is added to avoid oxidative degradation of the 8-oxo-dG site. The Greenberg laboratory has developed the synthesis of dinucleotide... 

Schmidt degradation of the acid 44 and fixation of the evolved carbon dioxide with barium hydroxide localized the label at C(17). Kuhn-Roth oxidation of 44 and Schmidt cleavage of the resulting two equivalents of acetic acid yielded the activities of both C(16) and its methyl substituent by comparison of the same sequence on the deacetylated y-lactams 39 and 40, respectively. Ozonolysis of acid 44 followed by reduction gave the epimeric lactam triols 45a and 45b, respectively. The usual Kuhn-Roth and Schmidt route offered independent determinations of C(ll) and C(5). A combined value for C(14) and C(15) could be calculated from the activity... 

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