Ox, spleen

Mills,164 using an ox-spleen preparation, was the first to observe that the pH-activity curve has two peaks, and that they show little shift in pH... 

Rapport, M. M., L. Graf, and N. F. Alonzo Immunochemical studies of organ and tumor lipids. VIII. Comparison of human tumor and ox spleen cytosides. J. Lipid Res. 1, 301 (1960). 

O-Seryl-A -acetyl-galactosamiiiidase activities have been described in preparations from ox spleen, snail, and earthworm (Bhargava el al., 1966 Bhargava and Gottschalk, 1967 Schauer and Gottschalk, 1968 Buddecke el al, 1969),... 

Mills in 1948 reported that the enzyme from ox spleen displayed two pH optima at 4.5 and 5.0-5.2, and he attributed the multiple optima to different proteins of identical specificity. Other investigators, however, (Levvy and Marsh, 1960) believed these multiple optima vrere due to a single enzyme in combination with different ions. The recognition of the existence of multiple forms of the enzyme which could be partially separated by ion-exchange column chromatography (Moore and Lee, 1960) produced a new field of j3-gIucuronidase. 

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