Ovary fixed tissue

The maternal ovaries are removed and examined at the time of necropsy and corpora lutea are counted. The corpus luteum (CL) is a transitory endocrine organ formed from the thecal and granulosa cells of the postovulatory follicle... Information on the number of copora lutea is useful in the interpretation of data on the viability of conceptuses (see below, pre-implantation loss). Corpora lutea counts can be conducted with fresh or fixed tissue, and are generally performed with the aid of a dissecting microscope. Minimal dissection of the ovarian tissue is required (13). 

Warnings and Cautionary Notes , 318 GFP Detection in Eiving Tissues Preparation of Samples, 318 GFP Detection in Fixed Tissues, 319 Protocol 17.1 Five Ovaries, 319... 

Toxicity tests were conducted in Chinese Hamster Ovary cells to determine the concentration of either Ni3S2 or NiS which affected cell plating efficiency following a 24 h exposure period. Proliferating cultures of Chinese Hamster Ovary cells were exposed to the nickel compounds for 24 h, and then the cells were trypsinized from the monolayer. Cell numbers were determined with a hemocytometer and 400 cells were plated to form colonies in 100 irni diameter tissue culture plates. The cells were incubated for about 9 days, fixed, stained, and the total number of surviving colonies in each plate were counted and expressed as a function of the total number of cells plated. 

Protocol 17.4 Live Larval, Pupal, and Adult Tissues, 322 Protocol 17.5 Using GFP Balancers to Genotype Embryos, 322 Protocol 17.6 Fixed Ovaries, 325 Protocol 17.7 Fixed Embryos, 524... 

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