Other Examples of Multienzymatic Cascade Processes, Including Bioreductive Reactions

Other Examples of Multienzymatic Cascade Processes, Including Bioreductive Reactions 

Along with the multienzymatic cascades presented in the previous sections, further systems using either ketoreductases or ADHs for the reductive step together with different enzymatic activities have been recently investigated and are worth mentioning. 

Isolated carbonyl reductases from either Candida magnoliae (CMCR reductase) or S. cerevisiae (Ymr266c reductase) were first employed for the biocatalytic 

A great effort has also been made for the development of multienzymatic cascade processes finalized to the preparation of enantiopure epoxides, important chiral synthones for the synthesis of drugs and biologically active compounds [2]. 

The irreversibility of the latter reaction allowed the formation of the corresponding P-azidoalcohols and P-hydroxynitriles in good to excellent yields and with perfect enantiopurity. As some ADHs, for example, ADH-A from R. ruber used in this study, suffer from inactivation from cyanide, addition of the nucleophile must be carried out after complete reduction of the a-chloro ketone substrate. 

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