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Origin replication element

Conjugative transposons are self-transmissible large DNA elements (up to 150kbp) located in the donor chromosome. After excision a circular intermediate is formed that is unable to replicate autonomously. It is nicked at a origin of transfer site and one strand is then transferred to the recipient cell. After generating a double-stranded circle the transposon integrates into the recipients chromosome. [Pg.386]

Research work with large genomes and the associated need for high-capacity cloning vectors led to the development of yeast artificial chromosomes (YACS Fig. 9-8). YAC vectors contain all the elements needed to maintain a eukaryotic chromosome in the yeast nucleus a yeast origin of replication, two selectable markers, and specialized sequences (derived from the telomeres and centromere, regions of the chromosome discussed in Chapter 24) needed for stability and... [Pg.314]

DNA molecule of E. coli. The three-letter names of genes and other elements generally reflect some aspect of their function. These include mut, mutagenesis dna, DNA replication pol, DNA po/ymerase rpo, RNA polymerase uvr, UV resistance rec, recombination dam, DNA adenine methylation lig, DNA ligase Ter, termination of replication and ori, origin of replication. [Pg.949]

Initiation The E. coli replication origin, oriC, consists of 245 bp it bears DNA sequence elements that are... [Pg.958]

D. The measurement of Li in brine (salt water) is used by geochemists to help determine the origin of this fluid in oil fields. Flame atomic emission and absorption of Li are subject to interference by scattering, ionization, and overlapping spectral emission from other elements. Atomic absorption analysis of replicate samples of a marine sediment gave the results in the table below. [Pg.471]

Muller WJ, Mueller CR, Mes AM, Hassell JA (1983), Polyomavirus origin for DNA replication comprises multiple genetic elements, J. Virol. 47 586-599. [Pg.71]

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See also in sourсe #XX -- [ Pg.326 ]



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