Organizers of IAC

Department of Chemistry and Materials Science Lawrence Livermore National Laboratory P. O. Box 808, L-268 Livermore, CA 94550 USA 

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Immunoaffinity procedures have also been developed to selectively extract corticosteroids from different sample matrices. Thus, Seymour et al. demonstrated the higher efficiency of the immunoaffinity methods compared with the conventional extraction procedures using organic solvents [177]. Immunosorbents have also been used for online procedures followed by HLPC-UV [178, 179], HPLC-APCI-MS [179,180], GC-MS [176,181], or capillary electrophoresis [182]. Poly(hydroxyethyl methacrylate) (HEMA) was evaluated as a support material for the anti-dexamethasone antibodies used in IAC. The online IAC-HPLC-MS allowed determination of dexamethasone and flumethasone in equine urine with LODs in the range 3-4 ng mL-1 [180]. The cross-reactivity values obtained in the ELISA and the recoveries of an IAC-HPLC procedure are presented in Table 7. Bagnati et al. developed an immunoaffinity extraction... 

Liquid-liquid extraction of OTA from the wine can be also performed (Zimmerli and Dick, 1996). A volume of 5 mL wine is mixed with 10 mL of aqueous solution containing 3.4% orthophosphoric acid (85%) and 1.18 g of NaCl, and extraction is performed twice with 5mL of chloroform each. The organic phases are combined, the solvent is removed under vacuum and the residue is redissolved in 5 mL of phosphate buffer solution (NaCl 120 mM, KC1 2.7 mM, phosphate buffer 10 mM, pH 7.4) prior to purification on IAC cartridge. 

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