Orcinol hydroxylase

Preliminary studies in our laboratory with orcinol hydroxylase, from a pseudomonad, suggested that the 1-dFAD-reconstituted enzyme would catalyze NADH oxidation, but 02 was reduced exclusively to H202. No oxygen transfer yielding trihydroxytoluene could be detected. Because of instability of both trihydroxytoluene oxygenation product and orcinol hydroxylase apoenzyme, the 1-dFAD studies were pursued next in collaboration with Massey, Husain, Ballou, and Entsch at University of Michigan with the most well characterized bacterial flavoenzyme hydroxylase, p-hydroxybenzoate hydroxylase (2, 26, 27). The apoenzyme was reconstituted stably with 1-deazaFAD... 

Nitrite reductase, Alcaligenessp. Nuclease A, S. aureus Oncomodulin, rat, native or apo-Orcinol hydroxylase, P. putida Ovalbumin... 

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