Optimization of column

Golshan-Shirazi, S. and Guiochon, G., Theory of optimization of the experimental conditions of preparative liquid chromatography optimization of column efficiency, Anal. Chem., 61, 1368, 1989. 

Recently, a combination of CFD and rate-based process simulation has been proposed as a way to link different scales. In the rate-based approach, the influence of the column internals on hydrodynamics and mass transfer is directly accounted via relevant hydrodynamic and mass transfer correlations. These correlations can be now obtained not only from real experiments, but also by application of CFD simulations, thus reducing the number of necessary hydrodynamic experiments. Such virtual experiments allow the optimization of column internals, even without really manufactured internals. 

In this contribution the focus will be on the optimization of column geometry for a fixed column distribution first, while the influence of the total number of columns as well as their distribution will then be shown for some examples. A more detailed description of this strategy has been published by Jupke (2004) and Jupke et al. (2002). 

If development as outlined meets the initial goal of separating the compounds, it is tempting to assume that method development is complete at this juncture. However, although both the column chemistry and the mobile phase have been selected, further optimization of column length, particle size, and flow rate is warranted (see Section III.E.b). If the separation provides the requisite k range, but does not adequately separate all components, the approach may be repeated using additional column types (phenyl or cyano columns). 

Chromatographic method development (the rapid optimization of column and mobile phase conditions for separation of the component of interest) may begin with a Cl8 or C8 packed column, and acetonitrile/water or methanol/ water as the eluent. As method development progresses, the initial choice of column might need to be reviewed if separation cannot be achieved by manipulation of the mobile phase conditions see Subheading 2.1.6.) and as more information is gained about the properties of the compound of interest,... 

Guillaume, Y. Guinchard, C. Study and optimization of column efficiency in HPLC Comparison of two methods for separating ten benzodiazepines. J.Liq.Chromatogr., 1994, 17, 1443-1459... 

The last topic to discuss in this chapter before going into economic optimization of column design is how to determine the diameter and length of the vessel. 

Fig 9. Separation of two substances on a packed column Optimization of column length... 

Development of Three Criteria for Optimization of Column Design... 

A General Description of Chromatography 762 26B Migration Rates of Solutes 765 26C Band Broadening and Column Etticiency 768 26D Optimization of Column Performance 775 26E Summary of Chromatographic Relationships 781... 

Van der Meeren, P, J. Vanderdeelen, M. Huys, L. Baert, Optimization of column loadability for preparative HPLC separation of soybean phospholipids, J. Am. Oil Chem. Soc., 1990,67, 815-820. 

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