Optimized Packed Columns for LC

In order to obtain numeric values for the optimized parameters, it is necessary to define a given separation and the equipment by which the sample is to be analyzed. 

Capacity Ratio (first eluted peak of the Critical Pair) (k ) Capacity Ratio (first eluted peak of the Critical Pair) (k ) Viscosity of the Mobile Phase (r]) 

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The different optimization equations derived in chapter 12 will then be used with these realistic chromatographic conditions in a simple optimization procedure. The conditions chosen are typical and might represent the average LC analysis. The values for (X) and (yp) are those estimated by Giddings [1] for a well-packed 

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