Optical trapping transfer

In one application, an epi-illuminated optical trap was used in conjunction with an inverted fluorescence microscope so that individual cells could be identified with FITC-conjugated antibodies and subsequently trapped for further micromanipulation (24). In the first part of the experiment, as a demonstration of a sorting technique, the trapped cells were transferred into a capillary tube, that was located in the Petri dish along with the cells. The optical trap was used to lift... 

One optical trap 830 nm Raman 413.1 nm Neuroglobin (Ngb) overexpressed in E. coli Oxidation state marker V4 at 1,361 and 1,374 cm Ngb over-expressed in E. coli is transferred back to deoxygenated state, even under oxygen-rich conditions. Ngb seems to respond to a reducing enzymatic system... 

This chapter reviews several techniques which combine the use of laser microbeams with antibodies to study molecular and cellular biology. An overview of the basic properties of lasers and their integration with microscopes and computers is provided. Biophysical applications, such as fluorescence recovery after photobleaching to measure molecular mobility and fluorescence resonance energy transfer to measure molecular distances, as well as ablative applications for the selective inactivation of proteins or the selective killing of cells are described. Other techniques, such as optical trapping, that do not rely on the interaction of the laser with the targeting antibody, are also discussed. 

It will be interesting to see how the balance shifts now that optically trapped ground-state molecules such as RbCs and KRb are becoming available, including the very recent announcement of optically trapped KRb produced by MA and Raman transfer... 

Similar time resolved techniques to study a host of transfer phenomena such as sensitization of luminescence, cross relaxation and optical trapping have been developed (Yen 1986b). Time resolved absorption or hole burning experiments are also possible but have been less common. In all of the above, the pulse length... 

In a series of studies, Hegarty et al. (1981,1982), Vial and co-workers (1979,1982) and Morgan et al. (1986a) have studied concentration quenching, optical trapping and other transfer processes in Pr and (Pr, Nd ) doped systems. They have obtained consistency of results with a minimum of adjustable parameters and have been able in the process to quantify the limits of validity of... 

Does T differ significantly from unity in typical electron transfer reactions It is difficult to get direct evidence for nuclear tunnelling from rate measurements except at very low temperatures in certain systems. Nuclear tunnelling is a consequence of the quantum nature of oscillators involved in the process. For the corresponding optical transfer, it is easy to see this property when one measures the temperature dependence of the intervalence band profile in a dynamically-trapped mixed-valence system. The second moment of the band,... 

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