Optical flow-through sensors based on a non-regenerable immobilized reagent

1 Optical flow4hrough sensors based on a non-regenerable bnmobilized reagent 

Most of the sensors using a consumable reagent are based on a chemiluminescence phenomenon that is revealed by a reagent such as trichlorophenyl oxalate (TCPO) and a fluorophore (usually a fluoranthene), or luminol. 

This type of sensor was adapted for biochemical applications by using an en2ymatic catalyst (an oxidase) immobilized on CPG and positioned prior to the detector, as shown in Fig. 3.34.C [242]. 

The above-described two-layer flow-cell was used for the determination of anions based on a quenching phenomenon. Table 3.4 gives the determination limits obtained by using various quenchers and a flow injection manifold in which the cell was inserted. The poor selectivity of quenching can be overcome by using a continuous separation technique e.g. HPLC), as in the determination of the anilines listed in Table 3.4. 

Hool and Nieman succeeded in immobilizing the reagent (luminol) on a support (CPG) located in the flow-cell of a chemiluminescence detector. For solubility reasons, the reaction between luminol and glutaraldehyde was 

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