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One-step conjugation

Amounts of proteins and glutaraldehyde used in the one-step conjugation procedure (Avrameas et al., 1978) ... [Pg.244]

Type of reaction C-N bond formation Reaction conditions Solvent-free, room temperature Synthetic strategy One-step conjugate addition Catalyst ZrOCl2.8H20/Montmorillonite KIO... [Pg.77]

Contrary to fraras-azoferrocene, the cis form exhibits one-step 2e oxidation waves, and its oxidation potential, E° = 0.03 V in Bu4NC104-benzonitrile, is more negative than that of the trans form (E() = 0.29 and 0.50 V vs. Ag/Ag ) by 0.3 V. These data imply that the 7r-conjugation ability and electron-withdrawing nature of the azo group is retarded in the cis form. [Pg.77]

The following procedure utilizes the one-step glutaraldehyde method. A two-step method may be used to somewhat limit polymerization of the conjugate (Chapter 20, Section 1.2). Varying the pH and the amount of glutaraldehyde added to the reaction can control the yield and molecular weight of the conjugates formed. [Pg.780]

Protocol for the One-Step Glutaraldehyde Conjugation of Ferritin to (Strept)avidin... [Pg.913]

Boorsma, D.M., and Kalsbeek, G.L. (1976) A comparative study of horseradish peroxidase conjugates prepared with a one-step and a two-step method. Histochem. Cytochem. 23, 200-207. [Pg.1049]

Biosynthesis is performed in one step by the enzyme L-histidine decarboxylase (HDC, E.C. 4.1.1.22). Histamine metabolism occurs mainly by two pathways. Oxidation is carried out by diamine oxidase (DAO, E.C. 1.4.3.6), leading to imidazole acetic acid (IAA), whereas methyla-tion is effected by histamine N-methyltransferase (HMT, E.C. 2.1.1.8), producing fe/e-methylhistamine (t-MH). IAA can exist as a riboside or ribotide conjugate. t-MH is further metabolized by monoamine oxidase (MAO)-B, producing fe/e-methylimidazole acetic acid (t-MIAA). Note that histamine is a substrate for DAO but not for MAO. Aldehyde intermediates, formed by the oxidation of both histamine and t-MH, are thought to be quickly oxidized to acids under normal circumstances. In the vertebrate CNS, histamine is almost exclusively methylated... [Pg.253]

See other pages where One-step conjugation is mentioned: [Pg.272]    [Pg.775]    [Pg.244]    [Pg.468]    [Pg.228]    [Pg.243]    [Pg.224]    [Pg.448]    [Pg.79]    [Pg.272]    [Pg.775]    [Pg.244]    [Pg.468]    [Pg.228]    [Pg.243]    [Pg.224]    [Pg.448]    [Pg.79]    [Pg.453]    [Pg.28]    [Pg.33]    [Pg.453]    [Pg.45]    [Pg.167]    [Pg.190]    [Pg.587]    [Pg.955]    [Pg.1061]    [Pg.166]    [Pg.29]    [Pg.25]    [Pg.955]    [Pg.427]    [Pg.82]    [Pg.247]    [Pg.32]    [Pg.35]    [Pg.182]    [Pg.235]    [Pg.278]    [Pg.757]    [Pg.776]    [Pg.779]    [Pg.798]    [Pg.475]    [Pg.8]    [Pg.487]   
See also in sourсe #XX -- [ Pg.243 ]



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