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Glutaraldehyde conjugation

Alkaline Phosphatase-Immunoglobulin Conjugate (Glutaraldehyde Protocol)... [Pg.138]

Table 6 Conjugation of an amino-containing hapten to a protein using the glutaraldehyde method... Table 6 Conjugation of an amino-containing hapten to a protein using the glutaraldehyde method...
Schiff base interactions between aldehydes and amines typically are not stable enough to form irreversible linkages. These bonds may be reduced with sodium cyanoborohydride or a number of other suitable reductants (Chapter 2, Section 5) to form permanent secondary amine bonds. However, proteins crosslinked by glutaraldehyde without reduction nevertheless show stabilities unexplainable by simple Schiff base formation. The stability of such unreduced glutaraldehyde conjugates has been postulated to be due to the vinyl addition mechanism, which doesn t depend on the creation of Schiff bases. [Pg.134]

Figure 1.105 Glutaraldehyde can undergo complex reactions with amine groups, resulting in aldehyde-containing derivatives that can be used in conjugation reactions. Figure 1.105 Glutaraldehyde can undergo complex reactions with amine groups, resulting in aldehyde-containing derivatives that can be used in conjugation reactions.
The reaction of glutaraldehyde with protein carriers and peptide haptens involves mainly lysine e-amine and N-terminal oc-amine groups. The conjugates formed are usually of high-molecular weight and may cause precipitation products. In addition, the orientation of the... [Pg.779]

The following procedure utilizes the one-step glutaraldehyde method. A two-step method may be used to somewhat limit polymerization of the conjugate (Chapter 20, Section 1.2). Varying the pH and the amount of glutaraldehyde added to the reaction can control the yield and molecular weight of the conjugates formed. [Pg.780]

Glutaraldehyde was one of the first and still is one of the most commonly used crosslinking agents available for creating antibody-enzyme conjugates. The crosslinking process using... [Pg.797]

Protocol for the One-Step Glutaraldehyde Conjugation of Ferritin to (Strept)avidin... [Pg.913]

See other pages where Glutaraldehyde conjugation is mentioned: [Pg.134]    [Pg.779]    [Pg.140]    [Pg.473]    [Pg.161]    [Pg.120]    [Pg.453]    [Pg.134]    [Pg.779]    [Pg.140]    [Pg.473]    [Pg.161]    [Pg.120]    [Pg.453]    [Pg.23]    [Pg.25]    [Pg.312]    [Pg.639]    [Pg.642]    [Pg.569]    [Pg.242]    [Pg.260]    [Pg.32]    [Pg.134]    [Pg.134]    [Pg.235]    [Pg.235]    [Pg.266]    [Pg.266]    [Pg.267]    [Pg.268]    [Pg.457]    [Pg.601]    [Pg.779]    [Pg.779]    [Pg.780]    [Pg.788]    [Pg.797]    [Pg.798]    [Pg.798]    [Pg.810]    [Pg.813]    [Pg.890]    [Pg.890]    [Pg.905]    [Pg.913]    [Pg.913]    [Pg.913]    [Pg.914]   
See also in sourсe #XX -- [ Pg.913 ]



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Glutaraldehyde

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