Oligotropha carboxidovorans

The carbon monoxide dehydrogenase of the aerobe Oligotropha carboxidovorans contains both Cu and Mo in the form of a cluster in which the Mo is bound to the thiol groups of molybdopterin cytosine nucleotide, and the Cu to cysteine residue in the form of a Cu-S-Mo(=0)OFl cluster (Dobbek et al. 2002). 

Carbon monoxide dehydrogenase Oligotropha carboxidovorans 137x2 FAD Mo molybdopterin... 

The CO dehydrogenase of the carboxidotrophic bacterium Oligotropha carboxidovorans is a molybdenum-containing iron-sulfur flavoprotein that catalyzes the oxidation of CO to CO2, generating a proton gradient across the cytoplasmic membrane (19, 20). 

Of the three members of the XO family, xanthine oxidase/dehydrogenases and aldehyde oxidoreductases catalyse the hydroxylation of carbon centres, whereas the third family member, the CO dehydrogenase from Oligotropha carboxidovorans, converts CO to CO2. This latter enzyme is a structural exception, in that it has a dinuclear heterometal [CUSM0O2H] cluster, with the sulfido ligand coordinated by the Cu centre (for the structure of this, see Chapter 4, Figure 4.12). 

The Mo-Cu CODH from Oligotropha carboxidovorans catalyzes the general reaction... 

XAS has also contributed substantially to our understanding of other members of this family, and in particular the novel carbon monoxide dehydrogenases. The first of these to be characterized was from the aerobic bacterium Oligotropha carboxidovorans. The enzyme catalyzes the reaction ... 

Zhang B, Hemann CF, Hille R (2010) Kinetic and spectroscopic studies of the [molybdenum-copper] CO dehydrogenase from Oligotropha carboxidovorans. J Biol Chem 285 12571-12578. doi 10.1074/jbc.M109.076851... 

---