Oligosaccharides heparin lyases

A comparison of size distribution of oligosaccharides released by heparin lyase digestion of pig-mucosal heparin with those calculated theoretically,2853 was consistent with a random distribution of cleavage sites within... 

A glycosaminoglycan isolated from lobsters was examined with heparin lyase and heparan sulfate lyase.275 It was degraded much less extensively than beef-liver heparin by the former, and not degraded by the latter, indicating a structure intermediate between those of heparin and heparan sulfate. Heparan sulfates from three species of mollusks219 were found to be resistant to heparin lyase action. With heparan sulfate lyase, similar oligosaccharides in different proportions were obtained. Comparison with the products from bovine-pancreatic heparan sulfate showed the same oligosaccharides. 

Polysaccharide lyases have been used to produce A -uronate containing disaccharides (Figure 4, la-lr) and higher oligosaccharides from heparin, heparan sulfate, chondroitin sulfates, dermatan sulfate, hyaluronan, and chemically modified GAGs. ... 

Figure 4. Depolymerization of heparin using polysaccharide lyase. The glycosidic linkage between a substituted glucosamine (X = H or SOa , Y = SO3 or CH3CO) and uronic acid (iduronic or glucuronic, X = SOa" or H) is cleaved eliminatively affording oligosaccharide products. The non-reducing end of each product formed contains a a-4,5 unsaturated uronic acid residue.

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