Oligonucleotides, sequence-specific probes

Saiki RK, Walsh PS, Levbnson CH., Erlich HA (1989) Genetic analysis of amplified DNA with immobilized sequence specific oligonucleotide probes. Proc. Natl Acad Sd USA 86 6230-6234. 

Bugawan, T.L., Begovich, A.B., and Erlich, H.A. (1990) Rapid HLA-DPB typing using enzymatically amplified DNA and nonradioactive sequence-specific oligonucleotide probes. Immunogenetics 32, 231-241. 

Holloway, J. W. Beghe, B. Turner, S. Hinks, L. J. Day, I. N. Howell, W. M. Comparison of three methods for single nucleotide polymorphism typing for DNA bank studies sequence-specific oligonucleotide probe hybridisation, TaqMan liquid phase hybridisation, and microplate array diagonal gel electrophoresis (MADGE). Hum. Mutat. 1999,14(4), 340-347. 

Metal NPs have received tremendous attention in the field of bio-analytical science, in particular the sequence-specific DNA detection [23,24]. This is attributed to their unique properties in the conjugation with biological recognition elements (e.g., DNA oligonucleotide probe) as well as in the signal transduction with optical [22,25], electrical [26], microgravimetric [27] and electrochemical [23,28-30] methods. 

In the multiplex-IPCR assays carried out by Joerger and Hendrickson, DNA of different lengths was used for separation of the PCR amplificates (see Fig. 4). Results of their experiments are discussed below in Section 3.5. The ability to discriminate between different DNA markers by length is nevertheless limited by the separation capabilities of the gel. For a large number of different DNA probes, a sequence-specific detection carried out, for example, by PCR-enzyme-linked oligonucleotide sorbent assay (ELOSA Section 2.2.2) should be preferable. 

---