Oligonucleotides phosphoramidite method

Oligonucleotide Synthesis (phosphoramidite method - Lessinger) Review Tetrahedron 1992, 48,2223... 

Modified oligonucleotides, synthesis by phosphoramidite method and application 93T6123. 

Oligonucleotide primers are synthesized with an Applied Biosystems 380A synthesizer by the phosphoramidite method T4 polynucleotide kinase (10 U//il) is obtained from Amersham (Amer-sham, UK)... 

A second method for synthesizing oligonucleotides with specific base sequences, using H-phosphonate monomers, has the advantage over the phosphoramidite method in that the monomers are easier to handle and phosphate protecting groups... 

Figure 7. Synthesis cycle in standard oligonucleotide synthesis using the phosphoramidite method. A 5 -OH-group is released by acid cleavage of the DMT-group B Substitution of the N(iPr)2-group by the 5 -OHgroup is catalyzed by l//-tetrazole C Unreacted 5 -OH-functional groups are capped by catalyzed acetylation D Cyanoethyl-phosphite is oxidized by iodine E Oligomer is cleaved from spacer by concentrated ammonium hydroxide solution and kept at 55 °C for 8 h for complete deprotection.

Two books have been published which contain much information on the use of tervalent phosphorus acid derivatives for the synthesis of oligonucleotide derivatives and analogues. L 2 a review on the synthesis of modified oligonucleotides by the phosphoramidite approach and their applications, and another review on the synthesis of specific ribonucleotides and unrelated phosphorylated biomolecules by the phosphoramidite method have appeared. A review on anellated heterophospholes gives an account on the preparation, characterization, and reactivity of such two-coordinate phosphorus compounds, e.g. (1). ... 

A large number of papers continue to appear on the preparation of base or sugar modified oligonucleotides by more or less standard phosphoramidite methods. Among base modified monomers prepared this year are the phosphoramidites of 2-amino-2 -deoxyadenosine (111), ... 

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