Oleate-auxotroph

Having successfully isolated an oleate-auxotroph, the Dutch group then consolidated this result by stabilizing the mutants by obtaining both... 

Perhaps of most Importance Is yeast s unusual ability among eukaryotes to be able to live anaerobically and to derive Its ATP by nonresplratory glycolysis. Under these conditions, as seen under the electron microscope, the mitochondria change morphologically to what are called promltochondrla (14), and the cells become auxotrophic for sterol (11) and unsaturated fatty acid such as oleate (15). Aerobically yeast derives Its sterol and unsaturates by biosynthesis, and for both types of compound there are biosynthetic steps Involving mixed function oxidases,, for the formation of squalene oxide... 

A dtsRl deletion mutant, auxotrophic to oleic acid,Tween 80 (oleate ester) or Tween 20 (laurate ester) overproduced glutamate. Glutamate overproduction was observed in biotin limitation and Tween 40 as it might lead to inactivation of the biotin enzyme... 

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