Ocean Eel Pout on the

The protein-ice modeling was conducted using the averaged simulation protein structure on the (100) ice plane. The protein was manually docked with the C-face of the Type III antifreeze protein on the (100) ice surface. This particular face of ice was chosen because of the three conseiwed residues (N14, T18, and Q44) which have been identified through mutation studies as being crucial for 

The process of docking followed by energy minimization was repeated many times. This was done to locate the lowest protein-ice complex. Once the lowest protein-ice complex was located, the antifreeze protein was then translated in the x and y directions by 0.5 A along the ice surface. After each translation a short energy minimization was performed. 

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