Nucleic acid sequencing analysis

Determined by protein or nucleic acid sequence analysis. ND, not determined. 

Human apoA-I is a major constituent of HDL, with an Mr of approximately 28,300, calculated from the known primary structure (Bl, B43). ApoA-I is initially synthesized as a 267-amino-acid precursor protein, pre-pro-apoA-I (G25, G26), containing an 18-amino-acid prepeptide and a 6-amino-acid propeptide [determined by nucleic acid sequence analysis of cloned apoA-I (L6), and by isolating the primary translation product of human intestinal apoA-I mRNA (G25)]. 

The structures of the rRNAs were generated and sequences compared using the University of Wisconsin Genetics Computer Group Nucleic Acid sequence analysis package29 and the University of Georgia Biological Structure and Sequence Computation Facility. 

Chapter 9 Applications - Nucleic Acid Sequence Analysis 05... 

Sequence similarity database searching and protein sequence analysis constitute one of the most important computational approaches to understanding protein structure and function. Although most computational methods used for nucleic acid sequence analysis are also applicable to protein sequence studies, how to capture the enriched features of amino acid alphabets (Chapter 6) poses a special challenge for protein analysis. 

Transfer RNA, tRNA, soluble RNA, sRNA. Low mol wt 23,000-27,000 approx 75-85 nucleotides. Each tRNA is specific for and binds with a particular amino acid more than one may exist for each amino acid. Performs three functions during protein synthesis binds with its specific amino acid recognizes the corresponding codon on mRNA and places the amino acid in the correct position for attach -ment to the polypeptide chain being formed binds the poly -peptide to the ribosome. First determination of total structure of a transfer RNA (yeast alanine tRNA) Holley et aL. Science 147, 1462 (1965). Reviews of structure and function Miura, Specificity in the Structure of Transfer RNA in fVogr, Nucleic Acid Res. Mol. BioL 6, 39-82 (1967) Cramer, Three-Dimensional Structure of tRNA , ibid. 11, 391-421 (1971) Nucleic Acid Sequence Analysis, S. Mandeles (Columbia University Press, New York, 1972) pp 256-280 Nishi-mura, "Transfer RNA Structure and Biosynthesis in MTP Int. Rev. Sci Biochem.. Ser. One vol. 6, K. Burton, Ed. (University Park Press, Baltimore, 1974) pp 289-322 A. Rich, V. L. Raj Bhandary, Ann. Rev. Biochem. 45, 805-860 (1976) P. F. Agris, The Modified Nucleosides of Transfer RNA, IT (A. R. Liss, New York, 1983) 220 pp. 

S. Mandeles, Nucleic Acid Sequence Analysis, Columbia University Press, New York, 1972. N. K. Kochetkov and E. I. Budovskii, Organic Chemistry of Nucleic Acids, Plenum, London,... 

Structure. The mononucleotides of RNA consist of ribose phosphorylated at C3, and linked by an N-gly-cosidic bond to one of four bases adenine, guanine, cytosine or uracil. Many other bases (chiefly methylated bases) also occur, but are less common (see Rare nucleic acid components). The mononucleotide units form a linear chain via 3, 5 phosphodiesler bonds (see Nucleic acids). Sequence analysis of RNA has become a standard technique. In many cases the amino acid sequences of proteins are predicted from the sequence of the corresponding mRNA (or DNA) because it is much easier to clone the nucleic acid than to isolate the protein. 

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