Nuclear isolation

The time necessary for nuclear isolation depends on the tissue type, extent of suboptimally fixed, partially autolyzed samples, and thickness of the tissue sections. 

The nuclear isolation method has been used for processing archival paraffin-embedded mammary tumors for monitoring estrogen expression and aneuploidy (Sabe et al., 1999). These two parameters have important diagnostic and prognostic significance in mammary tumors. 

Disruption often requires the use of hypotonic media and, where possible, non-ionic detergents. For nuclear isolation a buffer containing 20 mM Tris HC1, 1 mM EDTA and 1% Tween 80 or Triton X-100 is suitable and dithiothreitol and protease inhibitors may be... 

Nuclear isolation is a method by which large numbers of free intact nuclei are obtained. The method of isolation resulting in the highest yield of intact nuclei is dependent on the age and type of the particular tissue used. The method presented here5 has been demonstrated to give high yields of intact nuclei in maize, wheat, barley, oats, sorghum, and soybeans. 

Nuclei from normal somatic cells cannot be manipulated on a single-cell basis in the same way as giant nuclei from oocytes or polytene cells (see Fig. 3). Our own experience of mass nuclear isolation using the standard isolation procedures, which are well established for biochemical end points, showed that modifications... 

Because intact cells are impermeable to macromolecules, they have to be permeabilized before the NPC can be labeled with antinucleoporin antibodies. Alternatively, isolated nuclei or nuclear envelopes can be used. Therefore we first describe the various methods for cell permeabilization and nuclear isolation and then summarize the procedures used for immunolabeling. 

The proposal applies not only to isolated reactors, but also to other nuclear isolated facilities (for instance, spent fuel storage pools). 

Thickness of common materials required for nuclear isolation. 

Fig, 2. Nuclear isolation of arrays of cylinders by various materials. 

Xhomthwaite, J. X. Nuclear isolation medium and procedure for separating cell nuclei. U.S. Patent 4668618,1987. 

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