Novobiocin methods

Resistant by Kirby-Bauer disc diffusion method to penicillin G (P), Tetracycline (T), Clindamycin (C), Novobiocin (N), Kanamycin (K), Chloroamphenicol (Ch), Erythromycin (E). 

The International Standard Organization (ISO) reference method for isolation of E. coli 0157 (ISO 16654) from food recommends an enrichment in mTSB supplemented with novobiocin at 41.5°C for a total period of 18-24 h. In contrast, there is no standard method for enrichment of non-0157 E. coli, though a number of enrichment protocols have been reported for the isolation of a wide range of STEC or the recovery of some serogroups (Table 1). 

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