Normal-phase chromatography Nucleic acids

See also Liquid Chromatography Principles Normal Phase Reversed Phase Instrumentation. Nucleic Acids Chromatographic and Electrophoretic Methods Spectroscopic Methods. Optical Spectroscopy Stray Light. Ozone. Paints Water-Based Organic Solvent-Based. Peptides. Personal Monitoring Passive. 

A reversed-phase chromatography technique for the preparative separation of polar compounds has been demonstrated with the separation of a simple nucleoside mixture. A group separation of the 2 -deoxyribonucleoside, nucleotide and nucleobase constituents of normal and modified nucleic acids was achieved by gel permeation chromatography, and further separation was achieved on a hydrophilic acrylate polymer operating in the partition mode. Mononucleotides are selectively bound at low pH to Fe(ni) immobilized on agarose gel due to their free phosphate ester group, and can be recovered with a neutral eluant. Nucleosides and molecules with phosphate diester groups are not retained. ... 

A mixture of 14 normal and modified deoxyribonucleosldes and related nucleic acid constituents have been separated in less than 40 min by micellar electroklnetlc capillary chromatography (MECC) with u.v. detection. MECC is a new type of liquid chromatography which employs electroosmotically pumped micelle mobile phases (incorporating sodium dodecylsulphate), and offers the advantage of... 

---