Nootkatone biotransformation dothidea

The biotransformation from 1 to 2 was also examined using the plant pathogenic fungi B. dothidea and B. theobromae (a total of 31 strains) separated from fungi infecting various types of fruit, and so on. B. dothidea and B. theobromae were both inoculated and cultivated while stationary in Czapek-peptone medium (pH 7.0) at 30°C for 7 days. The same size of the substrate 1 was added to each medium and incubated for a further 7 days to obtain nootkatone (42%-84%) (Furusawa et al., 2005a). 

The expensive grapefruit aromatic nootkatone (2) used by cosmetic and ber manufacturers was obtained in high yield by biotransformation of (+)-valencene (1), which can be cheaply obtained from Valencia orange, by Chlorella species, fungi such as Mucor species, B. dothidea, and B. theobromae. This is a very inexpensive and clean oxidation reaction, which does not use any heavy metals, and thus, this method is expected to nd applications in the industrial production of nootkatone. 

Biotransformation of Nootkatone (2) by Fusarium culmorum AND Botryosphaeria dothidea... 

The biotransformation of nootkatone (2) was examined by the plant pathogenic fungus B. dothidea separated from the fungus that infected the peach. (+)-Nootkatone (2) was cultivated with B. dothidea (Peach PP8402) for 14 days to afford nootkatone diols (6 and 7) (54.2%) and 7a-hydroxynootkatone (16) (20.9%). Ratio of compounds 6 and 7 was determined as 3 2 by HPLC analysis of the thiocarbonates (12,13) (Noma et al., 2001b). Nootkatone (2) was administered into rabbits to give the same diols (6,7) (Asakawa et al., 1986 Ishida, 2005). 

The biotransformation of 2 by A. niger and B. dothidea resembled to that of the oral administration to rabbit since the ratio of the major metabolites 115- (6) and ll/ -nootkatone 11,12 diol (y) was similar. It is noteworthy that the biotransformation of 2 by F. culmorum affords stereospeci cally nootkatone-ll/f,12-diol (7) (Noma et al 2001b) (Figure 20.10). 

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