Nonspecific Antibody Binding to Tissue and Cells

Permeabilize Tissue and Cells to AUow Antibody Penetration 

Effects of Blocking Agents on Antibody Penetration. Combined Incubation Step. 

Antibodies can bind to cells in a highly specific fashion based on Fab-epifope binding. However, antibodies can bind to cells by nonspecific means fhaf will give incorrecf resulls. 

Immunocytochemistry, DOI 10.1007/978-f-4419-1304-3 5, Springer Science+Business Media, LLC 2010 

In tissues and cells there are four types of sites where an antibody can bind (Fig. 5.1), but only one is the correct site. Nonspecific binding occurs when an antibody binds by a mechanism other than by Fab-epitope binding. 

---