NOGen polymers

In another report PEG, which is a water-soluble, non-toxic, and non-immu-nogenic polymer, has been evaluated for its use as a non-biofouling coating for Si-based chips [912]. 

Figure 14.6 Two examples of NO-releasing (NORel) pol5mers where NO donor molecules (e.g., RSNOs or diazeniumdiolates) can be covalently or noncovalently incorporated into the PU. NO-generating (NOGen) polymers consist of immobilized catalysts that can generate NO from endogenous RSNO species.

Gupta, R.K. Griffin, P.J. Rivera, R. Siber, G.R. Development of an animal model to assess the immu-nogenicity of single-dose tetanus and diphtheria vaccines based on controlled release from biodegradable polymer microspheres. Dev. Biol. Stand. 1998, 92, 277-287. 

In another example, a hydrophilic star block copolymer was composed of a hyperbranched PEI core, a PLL inner shell and a PEG outer shell. Insulin, as a model protein, can be rapidly and efficiently encapsulated by the synthesized polymer in aqueous phosphate buffer at physiological pH. Complexation between PEI-PLL-Z)-PEG and insulin was demonstrated using native polyacrylamide gel electrophoresis. An in vitro release study by dialysis showed sustained release of the encapsulated protein at physiological pH, and an accelerated release when the pH was decreased. The insulin released from the star block copolymer retained its chemical integrity and immu-nogenicity. Successful in vitro uptake studies of enhanced green fluorescent protein into Ad293 cells mediated by PEI-PLL-Z)-PEG were also performed. ... 

Figure 14.7 Schematic of the assembled extracorporeal circulation (ECC) circuit and cross section showing generic coatings. Inner walls of the Tygon tubing and angiocatheters are coated with an active coat (containing the NORel or NOGen chemistry) or control coat (without NO chemistry), followed by a polymer top coat.

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