Nitrocephin disk

For some bacteria (eg, Haemophilus species), the susceptibility patterns of strains are similar except for the production of 13 lactamase. In these cases, extensive susceptibility testing may not be required, and a direct test for 3 lactamase using a chromogenic 3-lactam substrate (nitrocephin disk) may be substituted. 

There are a number of methods in use that directly detect the production of antimicrobial resistance in pathogens. /3 -Lactamase production can be detected rapidly and easily in the clinical laboratory with the use of nitrocephin disks. Nitrocephin is a chromogenic cephalosporin derivative that changes color on hydrolysis by /3-lactamase. Colonies from a growing bacterial culture can be touched to a disk, with /S-lactamase production noted within a few minutes. Although rapid and reliable, this method is limited to the assessment of strains of staphylococci, enterococci, H. influenzae, Moraxella catarrhalis, and N. gorwrrhoeae. The nitrocephin disk also cannot detect /3-lactam... 

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See also in sourсe #XX -- [ ]

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