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Nitrenes photoaffinity labeling, aryl azides

Photochemically reactive molecules have often been used as labels for specific sites in proteins and nucleic acids. Psoralen derivatives serve as relatively nonspecific photochemically activated crosslinking agents for DNA and double-stranded RNA.195 Aryl azides are converted by light to aryl nitrenes, which react in a variety of ways including insertion into C-H bonds (Eq. 23-27).200 201 In some cases UV irradiation can be used to join natural substrates to enzymes or hormones to receptors. For example, progesterone, testosterone, and other steroids have been used for direct photoaffinity labeling of their receptors.202 Synthetic benzophenones have also been used widely as photoactivated probes.203... [Pg.1297]

The reaction products from aryl azides in photoaffinity labeling experiments have not been fully characterized. Indeed the reactions of aryl nitrenes in solution remain a topic of current research. [Pg.32]

Studies of the photodecomposition of azido derivatives of PCB have attracted interest because of their potential use as photolabels in intracellular distribution studies.Polyfluorinated aryl azides are also of current interest as new reagents for photoaffinity labelling. The major products of photolysis of methyl 4-azidotetrafluorobenzoate in cyclohexane or diethylamine, for example, arise by insertion, a property which enhances its use in labelling. Singlet pentafluorophenyl nitrene, formed in an analogous fashion on irradiation of pentafluorophenyl azide, can be trapped by toluene as the insertion products (79) and (80). °... [Pg.381]

Photolysis of azides again leads to loss of N2, but in this case what is left behind is a ni-trene (Eq. 16.75). Photolysis of azides is the best route to nitrenes. Because of the high reactivity of nitrenes, aryl azides have been popular reagents for photoaffinity labeling (see the... [Pg.983]


See other pages where Nitrenes photoaffinity labeling, aryl azides is mentioned: [Pg.239]    [Pg.281]    [Pg.129]    [Pg.11]    [Pg.144]    [Pg.123]    [Pg.7]    [Pg.183]   
See also in sourсe #XX -- [ Pg.202 ]




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