Nipple Evolution of Lesions

Two and 3 2 hours after the test, we already observe outlines of spongiosis or some small true spongiosis.  

With the alkaline phosphatase reaction, this spongiosis does not yet seem to be invaded by the small lymphocytes (round cells) which appear on slides as black spots . Lymphocytes are disseminated in the dermis and the capillaries. Sometimes they are aligned underneath the epidermis, at the very place where there is already a spongiosis or the beginning of spongiosis (Fig. 8). 

Fisher and Cooke (1958) have previously observed signs of eczema very early in sensitized guinea pigs by a method according to Chase (2 hours following the test). 

After 5 to 6 hours spongiosis is clearly visible (Fig. 9). The infiltrate is sometimes minimal and sometimes considerable. The alkaline phosphatase reaction shows on the same slide both spongiosis invaded by lymphocytes ( inhabited spongiosis) and spongiosis not containing cells of the infiltrate ( non-inhabited spongiosis). 

After 9 hours there are numerous clusters of spongiosis in the epidermis and a very clear dermal infiltrate composed largely of lymphocytes (round cells). With the alkaline phosphatase reaction, we again observe that there are clusters of inhabited as well as non-inhabited spongiosis (Figs. 10 and 11). 

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