Neuraminidases purification

Stahl, W. L., and O Toole, R. D., 1972, Pneumococcal neuraminidase Purification and properties, Biochim. Biophys. Acta 268 480-487. 

Colominic acid, a homopolymer of iV-acetylneuraminic acid, has been mixed with soluble starch and cross-linked with epichlorohydrin to form a matrix suitable for the affinity chromatographic purification of neuraminidase. ... 

Purification of the neuraminidase from Vibrio cholerae 145 Studies of the effect of the chain-length on the adsorption 125 of albumin removal of albumin from sera Purification of Solanum tuberosum agglutinin (sta-lectin)... 

High neuraminidase activities have been shown to be produced by Arthrobacter ureafaciens, A. oxydans, and A. ausescens. Both colominic acid and N acetylneuraminic acid itself were shown to be effective as carbon sources. Affinity chromatographic purification on colominic acid cross-linked to starch by epichlorohydrin gave a product free from phospholipase C, glycoside hydrolases, and A-acetylneuraminic acid aldolase. 

Purification of neuraminidase (mol. wt. 4.8 x 10 , pH optimum 6.2) from Erysipelothrix rhusiophatiae has been reported. The enzyme exhibited highest activity against oligosaccharide (9). The rate of cleavage of (10) and of the a-(2 -> 8)-linkage of (11) were 20% of this. Gangliosides and colominic... 

The purification of neuraminidase from Streptomyces griseus via affinity chromatography on agarose-inunobilized fetuin has been reported. The enzyme was found to be a glycoprotein and selective chemical modication demonstrated that L-arginine residues are essential for full enzymic activity. 

Uchida Y, Tsukada Y, Sugimori T (1977) Distribution of neuraminidase in Arthrobacter and its purification by affinity chnunatography. J Biochem (Tokyo) 82 1425-1433... 

An extensive purification procedure, which included affinity chromatography on agarose derivatized with 4-nitrophenyloxamic acid, was necessary to isolate both forms of neuraminidase from porcine brain the two forms were then separated by chromatography on a hydroxyapatite-cellulose gel. The pH optima, maximum activities, and kinetic properties of the two forms differ and they are also activated by different cations. 

Purification of neuraminidase by affinity chromatography separation of two forms of neuraminidase 154... 

Aisaka, K., Igarashi, A., and Uwajima, T., 1991, Purification, crystallization, and characterization of neuraminidase from Micromonospora viridifaciens, Agric. Biol. Chem. 55 997-1004. 

Nees, S., Veh, R. W., and Schauer, R., 1975, Purification and characterization of neuraminidase from Clostridium perfringens, Hoppe-Seylers Z. Physiol. Chem. 356 1027-1042. 

Lock, R. A., Paton, J. C., and Hansman, D., 1988, Purification and immunological characterization of neuraminidase produced by Streptococcus pneumoniae, Microbiol. Pathog. 4 33-43. 

Scheibe, R., Hein, K., and Wenzel, K. W., 1990, Lysosomal p-galactosides from rat liver Purification, molecular forms and association with neuraminidase, Biomed. Biochim. Acta 49 547-556. 

Srivastava, P. N., and Abou-issa, H., 1977, Purification and properties of rabbit spermatozoal acrosomal neuraminidase, Biochem. J. 161 193-200. 

Tallman, J. F., and Brady, R, O., 1973, The purification and properties of a mammalian neuraminidase (sialidase), Biochim. Biophys. Acta 293 434-443. 

Taylor, G., Vimr, E., Carman, E., and Laver, G., 1992a, Purification, crystallization and preliminary crystallographic study of neuraminidase from Vibrio cholerae and Salmonella typhimurium LT2, J. Mol. Biol. 226 1287-1290. 

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