Neoglycoproteins binding

Biermann, L., Gabius, H.J., Denker, H.W. Neoglycoprotein-binding sites (endogenous lectins) in the fallopian tube, uterus and blastocyst of the rabbit during the preimplantation phase and implantation. Acta Anat., 1997, 160, 159-171. 

Fig. 3.—Inhibition of Binding of I-Labeled Asialo-orosomucoid ([ I]ASOR) to Rabbit-liver Membranes by Asialo-orosomucoid (ASOR), Orosomucoid (OR), BSA, and the Neoglycoproteins to Which 1-Thioglucopyranosides had been Attached by Amidin-ation (Glc-BSA). [The subscripts refer to the number of moles of attached 1-thiogluco-pyranosides per mol of BSA (taken from Ref. 97).]...

Comparison of Parameters of Neoglycoproteins Producing 50% Inhibition of Binding of [125I]-ASOR to Membranes12,07... 

Neoglycoproteins are suitable affinants for affinity chromatography of carbohydrate-binding proteins. As they can be obtained easily and inexpensively in large amounts, and as the methodology for attaching... 

The use of neoglycoproteins as affinants can be extended to the purification of such other classes of carbohydrate-binding protein as lectins, glycosylhydrolases, and glycosyltransferases. Some of the procedures that have been developed to prepare neoglycoproteins have been adapted to attach carbohydrates directly to Sepharose11,23,89,135, 177,178 and (2-aminoethyl)-Bio-Gel.59,81,87 These materials have been... 

Neoglycoproteins, liposomes, and glycopolymers have been successfully used to demonstrate that multivalency does indeed amplify carbohydrate-protein binding interactions by factors as high as thousands. However, by their very nature, these neoglycoconjugates have ill-defined chemical structures. They are heterogeneous in size and carbohydrate contents. Additionally, neoglycoproteins have been shown to... 

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