Neo gene

Fig. 2. Mutagenesis strategy. A neo gene replaced one-third of the ORF and was used as a positive selectable marker. The HSV thymidine gene (HSV-ffc) was used for negative selection. The vector was linearized with Xbal digestion. The targeted events were screened by Southern blot. Two Xbal sites are in the outside of the targeting vector (the 3 -end one is just at the end of the vector, which cannot be digested by Xbal if the vector is randomly inserted). An outside probe is located at the 5 -end. The genotype of the mice can be easily identified based on the size of the hybridized bands, 6.7 kb (mutant) vs 6 kb (wild-type).

Nrg1tmlLwr targeted mutation 1, Lorna W Role Disruption of the exon 7 by insertion of a nonsense mutation and an Xba1 site in the exon encoding the CRD domain. A lox-P flanked neo gene is also inserted into the intron following the CRD-encoding exon... 

Dulon D, Ryan AF. The bacterial Neo gene confers neomycin resistance to mammalian cochlear hair cells. Neuroreport 1999 10(6) 1189-93. 

Day 0 Split ES cells on 8.5-cm feeder plate as described in Section C. Inactivate fresh feeder and prepare eight 8.5-cm feeder plates. The culture of the feeder for inactivation should be started 1 week before electroporation. The fibroblasts should be prepared from embryos carrying the Neo gene, so that they... 

De Block, M., De Brouwer, D., Tenning, R, 1989. Transformation of Brassica napus and Brassica oleracea using Agrobacterium tumefaciens and the expression of the bar and neo genes in the transgenic plants. Plant Physiol. 91,694-701. 

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