Near-infrared protein content analysis

Models are constructed in analytics to describe the relationship between responses and factors. This is, for example, important for optimization of analytical methods on the basis of response surface methods (cf. Section 4.2). Models are also needed for cahbration of analytical methods. There, calibration of a single analyte in dependence on one or several wavelengths might be of interest. If, in the first example, the straight-line model would be adequate, for the second task of multiwavelength spectroscopy, multivariate approaches are needed. Calibrations in the case of unselective analytical methods must also be performed. These methods are termed simultaneous multicomponent analysis. In near-infrared (NIR) spectroscopy, the contents of water and protein in whole grain wheat are determined that way. 

T. Tajuddin, S. Watanab, K. Harada, S. Kawano. Application of near infrared transmittance spectroscopy to the estimation of protein and lipid contents in single seeds of soybean recombinant inbred lines for quantitative trait analysis. J Near Infrared... 

D. Bertrand, P. Robert, D. Lannay, and M. F. Devaux, Application of Principal Component Analysis to the Determination of Fat and Protein Content of Milk by Near Infrared Spectroscopy, Proc. Euro Food Chem. IV, Leon, Norway, 1987, pp. 519-523. 

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