National Centre for Biotechnology Information

ExPASy (Expert Protein Analysis System, www.expasy.ch) or the National Centre for Biotechnology Information (NCBI, www.ncbi.nlm.gov) websites. Both websites provide bioinformatics tools, links to sequence databases and extensive bibliographic resources. As an example of the wealth of information available on individual enzymes, at the time of writing a search based on nitrilase in the Entrez protein section of NCBI will recover more than 10000 references to nitrilase enzyme amino acid sequences. These can be rapidly screened online by organism, and the individual entries will have links to amino acid and gene sequence, relevant literature and information on protein features (such as conserved domains). 

High-speed identification from either PMF or MS/MS data can be achieved from non-redundant protein databases (Mann ef a/., 2001). These often include valuable annotations giving insight into functions of isolated and identified proteins. Many of these databases, such as NCBInr (National Centre for Biotechnology Information) and Swiss Prot (ExPASy Molecular Biology Server), can be used for protein identification. 

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