NaOH digestion

Fig. 3. Process flowsheet for P(3HB) production by recombinant E. coli with the recovery method of NaOH digestion...

After centrifugation of the TCA/acetone extract, nonquenching aliquots of the supernatant were counted by liquid scintillation, and the pellet digested overnight in 2N NaOH. Counts present in the TCA/acetone supernatant were taken to represent the uptake of label into the soluble amino acid pool, while solubilized counts present in the NaOH digest were taken to represent incorporation into TCA-insoluble protein. 

Normally, the raw sodium tungstate solution after NaOH digestion contains 200 g W04 /l, which is too high for the ion exchange process. The ion exchange capacity decreases with increasing WO3 concentration, as is shown in Fig. 5.11. 

Slurry from NaOH Digestion of Tungsten Ore Concentrates... 

Carbohydrates were extracted by Methanol, Chloroform, water (12/5/3, in 300 pi) and the water soluble fraction was analysed and quantified by HPLC. Starch was determined after treatment by 0.02 N NaOH, digestion by amyloglucosidase and enzymatic measurement of glucose. 

NaOH digestion-LLE (hexane)-HPLC-FD. Comparison with PAHs air levels. [381]... 

Three specific steps are taken to minimize the loss of ammonia (1) the solution is cooled to below room temperature before adding NaOH (2) the digestion flask is quickly connected to the distillation apparatus after adding NaOH and (3) the condenser tip of the distillation apparatus is placed below the surface of the HCI to ensure that the ammonia will react with the HCI before it can be lost through volatilization. 

Analytical Procedures. Oxygen difluoride may be determined conveniently by quantitative appHcation of k, nmr, and mass spectroscopy. Purity may also be assessed by vapor pressure measurements. Wet-chemical analyses can be conducted either by digestion with excess NaOH, followed by measurement of the excess base (2) and the fluoride ion (48,49), or by reaction with acidified KI solution, followed by measurement of the Hberated I2 (4). 

Kraft Process. The dominant chemical wood pulping process is the kraft or sulfate process. The alkaline pulping Hquor or digesting solution contains about a three-to-one ratio of sodium hydroxide, NaOH, and sodium sulfide, Na2S. The name kraft, which means strength in German,... 

Kraft pulping is a common process in the paper industry. Figure 8.4 shows a simplifled flowsheet of the process. In this process, wood chips are reacted (cooked) with white liquor in a digester. White liquor (which contains primarily NaOH, NaiS, Na2C03 and water) is employed to dissolve lignin from the wood chips. The cooked pulp and liquor are passed to a blow tank where the pulp is separated from the spent liquor weak black liquor which is fed to a recovery system for... 

Consid - the Kraft pulping process shown in Fig. 8.8 (Dunn and El-Halwa, 1993). The first step in the process is digestion in which wood chips, containing primarily lignin, cellulose, and hemicellulose, are cooked" in white liquor (NaOH, Na2S, Na2C03 and... 

CarboPak PA-1249 Sodium acetate-NaOH Dextrin digestion products... 

Type Addition temp (°C) NaOH Alloy (w/w) Digestion (°C/time) Washing process Relative activity... 

Sputum, for Nematode larvae or Paragonimus eggs Break up mechanically or digest 1 part sputum plus 5 parts 3% NaOH for 1 h. Centrifuge, and preserve the sediment in an equal volume of 10% buffered Formalin. 

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