Nanoparticle-protein complex

Lynch, I. et al. (2007) The nanoparticle-protein complex as a biological entity a complex fluids and surface science challenge forthe 21st century. Advances in Colloid and Interface Science, 134—135, 167-174. 

Nishikawa T., Akiyoshi K., Sunamoto J. Supramolecular assembly between nanoparticles of hydrophobized polysaccharide and soluble protein complex-... 

Indirect amplified electrochemical analysis of aptamer-protein complexes has employed nanoparticles as labels for the development of electrochemical aptasensors. The use of metal nanoparticles as tracers for the analysis of nucleic acid... 

As an example, it is useful to recall protein solutions. Protein molecules can be quite large for example, a lysozyme molecule is an ellipsoid with dimensions 3x4x5 nm, similar to nanoparticles (they are in fact nanoparticles). Protein aqueous solutions have seen significant smdy in the recent past because it is desirable to form protein crystals from solution for strucmral analysis. Such a system contains not only the protein molecule and the water solvent but usually dissolved ions which dissociate and a variable pH. Moreover, the protein molecule may have a variety of surface states that affect its interaction with other protein molecules as well as the water. The lesson here is that often the system is too complex and an effective inter-particle interaction potential must be prescribed. Such a procedure often works because the number concentration of the large protein molecules or nanoparticles is far less than that of the other constituents. 

The cooperative effect of charges and hydrophobic functions on protein complexation was also investigated in another study using poly 9,9-bis[6 -(ArfiST,N-trimethylammonium)he g l](fluorophenylene) bromide /human serum albumin nanoparticles. In this case, the formation of assemblies with high affinity was the result of a combination of electrostatic interactions between the cationic side chains of the polymer and the negatively charged surface of the protein, in addition to the hydrophobic interactions between the polymer backbone and the hydrophobic patches of the protein. 

Chitosan-heparin polyelectrolyte complex nanoparticles Protein delivery [236]... 

Teng Z, Luo Y, Wang Q (2013a). Carboxymethyl chitosan-soy protein complex nanoparticles for the encapsulation and controlled release ofvitamin D3. Food Chem, 141,524-532. 

Akiyoshi K, Kobayashi S, Shichibe S et al (1998) Self-assembled hydrogel nanoparticle of cholesterol-bearing pullulan as a carrier of protein drugs Complexation and stabilization of insulin. J Control Release 54 313-320... 

S. Xu and M. Yonese, in Charge Densities and Nanoparticle Formation of Complexes Composed of Acid Polysaccharides and Protein, Nagoya, Japan, 1998, p. E1076. 

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