N guanidine

N-guanidine-acetamide -daunomycin -co-ch3 N-guanidine- acetarride-daunosamine... 

Dihydro daunomycin caused a remarkable reduction of foci formation, complete inhibition being obtained at 0.1 pg/ml. Foci formation was more sensitive to this compound than cell proliferation. N-guanidine daunomycin had no cytotoxic activity at the doses tested, while weakly inhibiting foci production. 

Daunomycin and adriamycin at 10 jug/reaction mixture (0.25 ml) inhibit the reverse-transcriptase reaction by 60—70%. The dihydro derivative is also quite effective whereas the N-guanidine derivative has moderate activity. The N-acetyl derivative was completely ineffective in the MSV (M) and FLV systems but moderately inhibited the RSV system. 

Figure 10.- The N R spectra of B. Lichenlforais cell walls stripped of teichoic acid and techuronic aci A in H2O (A)/ in 6 N guanidine hydrochloride (B), in 6 N guanidine hydrochloride at 27 (C) and 60 C (D) after boiling in 4% SDS for 5 minutes to remove non-covalently bound protein. Spectral conditions as in Figure 1.

Kingston RE, Chomczynski P, Sacchi N. Guanidine methods for total RNA preparation. Curr Protoc Mol Biol 2001 May Chapter 4 Unit4 2. Ribaudo R, Gilman M, Kingston RE, Chomczynski P, Sacchi N. Preparation of RNA from tissues and cells. Curr Protoc Neurosci 2001 May Appendix 1 Appendix II. 

S. Levmore, PTir Blast Parameters and Other Characteristics of Nitroguanidine and Guanidine Nitrate, TR 4865, PTA, Dover, N.J., 1975. 

The methacrylic backbone structure makes the spherical Toyopearl particles rigid, which in turn allows linear pressure flow curves up to nearly 120 psi (<10 bar), as seen in Fig. 4.45. Toyopearl HW resins are highly resistant to chemical and microbial attack and are stable over a wide pH range (pH 2-12 for operation, and from pH 1 to 13 for routine cleaning and sanitization). Toyopearl HW resins are compatible with solvents such as methanol, ethanol, acetone, isopropanol, -propanol, and chloroform. Toyopearl HW media have been used with harsh denaturants such as guanidine chloride, sodium dodecyl sulfate, and urea with no loss of efficiency or resolution (40). Studies in which Toyopearl HW media were exposed to 50% trifluoroacetic acid at 40°C for 4 weeks revealed no change in the retention of various proteins. Similarly, the repeated exposure of Toyopearl HW-55S to 0.1 N NaOH did not change retention times or efficiencies for marker compounds (41). 

One such compound, bropirimine (112), is described as an agent which has both antineo-plastic and antiviral activity. The first step in the preparation involves formation of the dianion 108 from the half ester of malonic acid by treatment with butyllithium. Acylation of the anion with benzoyl chloride proceeds at the more nucleophilic carbon anion to give 109. This tricarbonyl compound decarboxylates on acidification to give the beta ketoester 110. Condensation with guanidine leads to the pyrimidone 111. Bromination with N-bromosuccinimide gives bropirimine (112) [24]. 

Chemical Name N-cyano-N -methyl,N"-[2-[ [(5-methyl-1 H-imidazol-4-yl)methyl] thio] ethyl] guanidine... 

N sodium hydroxide solution (5 ml) is added to a stirred suspension of S-methyllsothiosemi-carbazide hydroiodide (2.33 g) and hydroxylamine hydrochloride (0.70 g) In water (6 ml) and stirred for 48 hours. The solution is evaporated In vacuo to provide 1 -amino-3-hydroxy-guanidine. One-third of the residue is dissolved in 16 ml of ethanol and 2,6-dichlorobenzalde-hyde (0.6 g) Is added to this solution. The reaction mixture is then stirred for 48 hours. The solution is then evaporated in vacuo and the residue dissolved in ether (30 ml) and in hydrochloric acid (30 ml). The aqueous phase is rendered alkaline with 2 N sodium carbonate solution and extracted with ether. The ether layer is dried with sodium sulfate and evaporated. 

The above keto-nitrile (15 grams) was methylated with a solution of diazomethane in ether. (The diazomethane solution was prepared using 20 grams of N-nitrosomethylurea.) The ether and excess diazomethane were evaporated on the steam bath and the oil dissolved in ethanol (50 ml). To this was added a solution of guanidine in ethanol (100 ml) (prepared from 8.1 grams of the hydrochloride). The solution was refluxed for 5 hours, the alcohol removed and the residue treated with 5N sodium hydroxide. The insoluble material was then filtered. After purification by precipitation from dilute acetic acid with sodium hydroxide and by recrystallization from ethanol the product formed clear colorless needles (8.0 grams), MP 218°-220°C as described in U.S. Patent 2,602,794. 

For purification, the product is recrystallized from 15 times the amount of a 9 1 mixture of acetone and water. The resulting N -[p-amino benzene sulfonyl]-N -(4,5-dimethyl-oxa-zolyl-(2)] guanidine is obtained as colorless crystals having a MP of 233 to 236°C. 

The reaction of N-amino heterocycles 759 and 760 with diaryl carbodii-mide gave triazolotriazine 761 in good yield. In some cases the intermediate guanidines are isolated which by thermal or basic treatment cyclized (86H3363 89H1607) to neutral or mesoionic compounds. 

Borane, 1-methylbenzylaminocyanohydropyrrolyl-, 3, 84 Borane, thiocyanato-halogenohydro-, 3,88 Borane, trialkoxy-amine complexes, 3, 88 Borane, triaryl-guanidine complexes, 2,283 Borane, trifluoro-complexes Lewis acids, 3,87 van der Waals complexes, 3, 84 Borane complexes aminecarboxy-, 3,84 aminehalogeno-, 3, 84 amines, 3, 82, 101 B-N bond polarity, 3, 82 preparation, 3, 83 reactions, 3, 83 bonds B-N, 3, 88 B-O, 3, 88 B-S, 3, 88 Jt bonds, 3, 82 carbon monoxide, 3, 84 chiral boron, 3, 84 dimethyl sulfide, 3, 84 enthalpy of dissociation, 3, 82... 

Bicychc pyrazinones foimd in several natural products were synthesized via Michael addition of heterocyclic amines to nitro olefin followed by reduction/cyclization of the nitro group of the adduct [20] (Scheme 5). Further elaboration of the C-6 methoxycarbonyl group in pyrazinone to the n-propyl guanidine group could result in the synthesis of indoloperamine. 

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