N-acetyl-asparagine

PhsSnflV)] complexes of N-Ac-Gly, Af-acetyl-leucine, N-acetyl-asparagine, and N-Ac-L-Tyr were prepared by two procedures and characterized by means... 

We selected two samples for the sequence comparisons. The first sample was a 1 mM ubiquitin protein dissolved in 90% H2O with 10% D2O with 1 mM DSS as an internal reference. The sample was deliberatively shortened to 550 uL to make the shimming less effective. The sample was symmetrically shimmed, but only dovm to a linewidth (50% height) of 1 Hz. This sample was used to evaluate the solvent amplitude after suppression and to give insight into the relative effect (if any) of the solute signals on an 800 MHz instrument equipped with 5 mm HCN cold probe. The results of the tests were discussed in Section 4.1. The second sample was a 10 mM N-acetyl-asparagine in 98% D2O (600 uL) also symmetrically shimmed with a final width at 50% height of 0.77 Hz. This sample was ideal in terms of efficient solvent suppression and was used to compare and evaluate the baseline characteristics and off-resonance effects of the sequences on solute suppression (Section 4.2). 

Figure 12 Bandwidth, artefacts, and baseline. Pulse sequences previously tested in Figure 11 were applied to a sample of 10 mM N-acetyl-asparagine (98% D2O) in order to evaluate the effects of the various suppression methods on exchangeable atoms (amides), resonances close to the solvent (HaS), and extremely intense peaks (acetyl group).

Figure 5.4. Schematic representation of glycosylated IgG. Asn, asparagines Fuc, fucose GIcNAc, N-acetyl-glucoseaimine Man, Mannose Gal, galactose Sia, sialicacid.

Mono-a-(l— 6) substitution of Man-3 in 54 is evident from the relative intensities of several structural-reporter-group signals (in the spectrum of the mixture) that are markers for the type of mono-substitution of Man-3 it may most readily be inferred from the N-acetyl singlets of GlcNAc-2 at 8 2.095 (lower branch) and 2.090 (upper branch), occurring in the ratio of 7 1. As 47, the main component of the mixture, is a lower-branch structure, whereas the other minor component, 46, is an upper-branch glyco-asparagine (see earlier), it... 

Fig. 43.—Structural-reporter-group Regions of the Resolution-enhanced, 500-MHz, H-N.m.r. Spectrum of a Mixture Containing Compounds 60 and 58 in the Ratio of 9 1. [In contravention of the usual experimental conditions (see Section IV), the glyco-asparagine solution used for recording this spectrum had pD 2. The bold number and letters in the spectrum refer to the corresponding residues in the structures, and the italic numbers refer to the compounds in the mixture. The relative-intensity scale of the N-acetyl-proton region (see insertion) differs from that of the other parts of the spectrum, as indicated.]...

Here is N- acetyl - galactosami ne attached to an asparagine residue as an JV-glycoside. 

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