Myosin heavy chain staining

FIGURE 19.2 Smooth-muscle myosin heavy chain stains myoepithelial cells in a fibroadenoma. 

Because myoepithelial cells are not always readily identifiable on routine hematoxylin- and eosin-stained sections, many immunohistochemical methods have been used to highlight an intact myoepithelial cells layer. Recent studies have reported CD10 and smooth muscle myosin heavy chain (SMMHC) expression in... 

Immunohistochemical analyses were performed using antibodies recognizing smooth muscle actin (SMA), troponin-T (Trop-T), troponin T-C (Trop T-C), myosin heavy chain (MHC), muscle actin (MA), a-actinin, a-SR-1, desmin, connexin-43, fibroblasts (all antibodies from Dako, Denmark). For fluorescent immunohistochemistry the AlexaFluor 594 goat anti mouse secondary antibody (Molecular Probes, Leiden, The Netherlands) was used. In addition, cell nuclei were stained using DAPI. 

Fig. 4 Utilization of Morphology Explorer to determine optimal time point of differentiated myotubes. Images of human skeletal muscle cells stained by immunofluorescence of a-myosin heavy chain (a-MHC), Titin, Vimentin, and nuclei by Hoechst 33342 were acquired at 2 days before cell confluence (-2), at cell confluence (0), and at day 3,7,10, and 14 post cell confluence (a-r) Quantitative readouts of cell culture features, obtained from four selected output parameters from the Morphology Explorer bio-application, showed that peak of differentiated myotubes were obtained on day 3 post confluence (s-v)...

FIGURE 19.1 p63 (A) and smooth-muscle myosin heavy chain (B) stain myoepithelial cells (MEQ of a breast lobule. 

FIGURE 19.6 Approximately 5% of morphologically identifiable DCIS may not show myoepithelial cells. This case of cribriform and papillary DCIS (A) shows lack of staining with p63 (B) and smooth-muscle myosin heavy chain (Q. Collagen type IV demonstrates strong continuous staining around tumor nests confirming the in situ nature of the lesion (D). 

FIGURE 19.13 Initial microscopic impression of tumor nest is that myoepithelial cells are present, manifested by the presence of smooth-muscle myosin heavy chain (A). Closer inspection reveals staining of vessels (note lumens) around tumor nests (B). No im-munostaining for p63 documents lack of myoepithelial cells in this case (C). 

Figure 15.4 Printed primary human myoblasts and rat tenocytes to generate muscle-tendon tissues for substance testing. The cells were printed and immunostained for myosin heavy chain (green) in (a) and collagen I (green) in (b) for muscle and tendon tissues, respectively. In (c) the close-up of (a) shows the characteristic muscle striation and multinucleated cell bodies of differentiated myoblasts. Nuclei are stained in blue with (4, 6-diamidino-2-phenylindole). In (d) the close-up of (b) shows the characteristic mature tendon collagen I pattern around the cell nuclei, which are stained in red with propidium iodide. Scale for (a) and (b) = 1 mm. Scale for (c) and (d) = 20 pm.

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