Mutation scanning

Gasser, R.B., Monti, J.R., Bao-Zhen, Q., Polderman, A.M., Nansen, P. and Chilton, N.B. (1998) A mutation scanning approach for the identification of hookworm species and analysis of population variation. Molecular and Biochemical Parasitology 92, 303—312. 

Conole, J.C., Chilton, N.B., Jarvis, T. and Gasser, R.B. (2001) Mutation scanning analysis of microsatellite variability in the ITS-2 (precursor rRNA) for three species of Metastrongylus (Nematoda Metastrongyloidea). Parasitology (in press). 

Gasser, R.B. (1997) Mutation scanning methods for the analysis of parasite genes. InternationalJournal for Parasitobgy 27, 1449-1463. 

Zhu, X.Q. and Gasser, R.B. (1998) SSCP-based mutation scanning approaches to fingerprint sequence variation in ribosomal DNA of ascaridoid nematodes. Electrophoresis 19, 1366-1373. 

Olekhnovich, I.N., and Kadner, R.J. (2002) Mutational scanning and affinity cleavage analysis of UhpA-binding sites in the Escherichia coli uhpT promoter. /. Bacteriol. 184, 2682-2691. 

The principal factor influencing the sensitivity of this technique to base changes is the combination of the mismatched bases. Greater mobility differences (relative to the homoduplex) occur in the order of G G/C C > A C/T G A G/T C > A A/T T. This and other mutation-scanning methods are useful when a wide variety of sequence alterations might be present. Particularly when most of the samples tested are wild-type ("normal )) it is more economical to scan for the presence of mutations before performing specific genotyping or DNA sequence analysis. 

Highsmith WE Jr, Nataraj AJ, Jin Q, O Connor JM, Ei-Nabi SH, Kusukawa N, Garner MM, Use of DNA toolbox for the characterization of mutation scanning methods. II evaluation of single-strand conformation polymorphism analysis. Electrophoresis 1999 20 1195-203. 

Feng J, Yan J, Buzin CH, Sommer SS, Towbin JA. Comprehensive mutation scanning of the dystrophin gene in patients with nonsyndromic X-linked dilated cardiomyopathy. J Am CoU Cardiol 2002 40 1120-4. 

Hu M, D AmelioS, Paggi L, Gasser R (2001). Mutation scanning for sequence variation in three mitochondrial DNA regions for members of the Contracaecum osculatum (Nematoda Ascaridoidea) complex. Electrophoresis, 22 1069-1075. 

Buch, J.S., Rosenberger, R, Highsmith, W.E., Kimball, C., DeVoe, D.L., Lee, C.S., Denaturing gradient-based two-dimensional gene mutation scanning in a polymer microfluidic network. Lab Chip, 5,392-400, 2005. 

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