Affinity cleavage

Olekhnovich, I.N., and Kadner, R.J. (2002) Mutational scanning and affinity cleavage analysis of UhpA-binding sites in the Escherichia coli uhpT promoter. /. Bacteriol. 184, 2682-2691. 

One critical exception is the sapphyrin-ethylenediaminetetraacetic (EDTA) conjugate (23). Previous studies of small molecule-EDTA conjugates have shown that these species, in the presence of iron(II), Oj, and a suitable reducing agent, can effect the cleavage of the DNA backbone. In turn, so-called affinity cleavage can be used to elucidate the specificity of the small molecule-DNA interactions. It was thus considered worth making 23. ... 

From affinity cleavage analysis, the stabilities of the base triplets were found to decrease in the order P1GC> >P1CG> >P1AT, Pl TA at pH 7.4, thus demonstrating a sequence-selective binding of Pl to GC. ODNs containing Pl... 

Meares CF, Datwyler SA, Schmidt BD, Owens J, Ishihama A Principles and methods of affinity cleavage in studying transcription. Meth Enzymol 2003, 371 82-106. 

Patchornik G, Goldshleger R, Karlish SJ. The complex ATP-Fe< > serves as a specific affinity cleavage reagent in ATP-AAg< sites of Na,K-ATPase altered ligation of Fe< > (Mgi >) ions accompanies the E(1) —E(2) conformational change. Proc Natl Acad Sci U S A 2000 97 11954-11959. 

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