Mutation, Mutants

Shiau SY, Huff RA, Wells BC, et al. 1980. Mutagenicity and DNA-damaging activity for several pesticides tested with Bacillus subtilis mutants. Mutat Res 71 169-179. 

S627A <2> (<2> catalytic domain mutant, mutation of potential phosphorylation site, no phosphorylation of the mutant, reduced activity, increased Km values for the substrates, but higher activity than the unphosphorylated wild-type enzyme [21]) [21]... 

McDougall, K.J. Lemontt. J.F. (1979) Effects of spermine on the detection of induced forward mutation at the can locus in yeast evidence for selection against canavanine-resistant mutants. Mutat. Res.. 63. 21-34... 

Substitute the amino acid at position 46 of object wt with a proline (P). mutate sequence wt 5 E mutant2 mutate sequence mutant2 32 P mutants mutate sequence mutant3 47 L mutant4... 

Fig. 2. Biochemical classification of the nia mutants, mutated domains and intragenic complementation. A collection of Nicotiana plumbaginifolia nia mutants (defective for NR apoenzyme) was tested for NR protein and activities (Cherel et al., 1990) and checked for in vivo and in vitro intragenic complementation (Pelsy Gonneau, 1991). NR protein amounts were measured by a sandwich ELISA test using as first antibody the anti-corn NR monoclonal antibody 96.9.25 (Ch6rel eta/., 1985) shown to be directed against the haem domain (M. Kavanagh, personal communication Meyer etal., 1991). The result is shown here as + or when a positive or negative ELISA test, respectively, was observed. Many class 4 mutants, most probably frameshift and deletion mutants, complement class 3 but not class 2 mutants.

Bischoff DS, Slavicek JM (1997b), Phenotypic and genetic analysis of Lymantria dispar nucleopolyhedrovirus few polyhedra mutants mutations in the 25K FP gene may be caused by DNA replication errors, J. Virol. 71 1097-1106. 

Benyajati C, Place AR, Sofer W. 1983. Formaldehyde mutagenesis in Drosophila Molecular analysis of ADH-negative mutants. Mutat Res 11 1-7. 

Teranishi K, Hamada K, Watanabe H. 1975. Quantitative relationship between carcinogenicity and mutagenicity of polyaromatic hydrocarbons in Salmonella typhimurium mutants. Mutat Res 31(2) 97-102. 

An extracellular peptide, EDF-A is required for sporulation of B. subtilis [202]. Its production is cell-density dependent. Thus, dilute bacterial suspensions sporulate poorly when decoyinine is added or the population is shifted down, unless EDF-A, present in the extracellular medium of high-density preparation, is added. The pheromone is destroyed by pronase and is dialyzable and heat-resistant. EDF-A production is defective in spoOA or spoOB mutants. Mutations in abrB, which suppress many of the pleiotropic phenotypes in spoOA mutants - except sporulation - restore production of EDF-AA. 

In the C, elegans embryo, molecular analysis of three mutants revealed that they encode ptroteins similar to pore (mom-1), mom-2, and frizzled (fz), a Wnt receptor (mom-5). Similar to the pan mutant, mutation of pop-1, an HMG box protein results in an opp>osite effect of mom mutations both EMS daughters adopt the E fate and produce exclusively gut (Wodarz Nusse, 1998). 

G. M. Williams, C. Tong, and J. J. Berman, Characterization of analog resistance and purine metabolism of adult rat-liver epithelial cell 8-azaguanine-resistant mutants, Mutat. Res. 49, 103-115 (1978). 

Injected stage Quantity of injection (% X ml) Dose per capita (Mg) Total number observed Number of mutants Mutation frequency (10 / g/locus) Exptl. season ... 

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