Mutant overexpression

Two approaches have been used to reduce secondary consequences of Arf6 mutant overexpression. The first is to reduce expression time. Whereas Arf6Q67L expression accelerated clathrin-independent endocytosis at early times following transfection, it inhibited this pathway at longer expression times (Naslavsky et al., 2004). Expressing Arf6 mutants with... 

An important validation of the mice that overexpress human mutant 3APP as a platform for testing therapeutics targeting 3-peptide deposition has been provided by the Elan company, using their PDAPP mouse [146]. Immunization of the mice, either at an early age or after plaques had formed, resulted in clearance of immunoreactive plaques and peptide from the subjects brains. Although the elucidation of the mechanism explaining... 

Chia There is another experiment that is relevant to your question. If Pins is overexpressed in the epithelial cells in a Pins- mutant, there is no longer any apical Insc. But this distribution is bizarre it is in the cytoplasm, but it is preferentially in the apical cytoplasm, as if apical Baz were trying to recruit Insc. One might speculate that the transient Baz—Insc complex can t be stabilized in the absence of Pins, so Insc keeps falling off. In this situation you don t get spindle reorientation. 

The Fur protein regulates iron uptake systems in many Gram-negative bacteria. The striking phenotype of the first fur mutants isolated was the overexpression of the outer membrane receptors for siderophore iron transport. In addition, excretion of siderophores under iron-rich growth conditions was observed in these mutants, indicating that the biosynthesis of siderophores is also regulated by Fur. 

An increasing number of G proteins and RGS proteins have been deleted or overexpressed in genetic mutant mice. Not surprisingly, some of these mice exhibit complex behavioral abnormalities and in some cases abnormal responses to psychotropic drugs. These data further highlight the importance of G protein signaling in the brain in health and disease and open new paths for investigation in the years ahead. 

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