Muscle from frozen-thawed meat

Such membrane damage has been observed by freezing and thawing of tissue, for example, of muscle tissue, in which the isoenzymes of glutamate oxalacetate transaminase (GOT) bound to mitochondrial membranes are partially released and found in the sarcoplasm. The pressed sap collected from fresh unfroze meat has only sarcoplasm enzymes, while the frozen and thawed meat has, in addition, the isoenzymes derived from mitochondria. The GOT isoenzymes can be separated by electrophoresis (Fig. 12.41). This procedure is also apphcable to fish. 

---