Musca bioassay activity

A similar, but more sensitive, bioassay using larvae of Musca domestica was developed [174-177], This bioassay is still finding application today [140,178], Table (1) summarises the biological activities of ecdysteroids in this bioassay. 

In yet another structure-activity investigation, Crombie and Denman [80] demonstrated that the synthetic 10-Z isomer corresponding to pipercide, 50(n=4) was more active in bioassays against the common housefly, Musca domesiica, than the natural, all-E amide, both with respect to kill and knockdown. Some parallels to this kind of structure-activity relationship are found also in the aliphatic amide series [80]. 

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