Multireactant Enzymes

Cook PE, Cleland WW. Mechanistic deductions from isotope effects in multireactant enzyme mechanisms. Biochemistry 1981 20 1790-1796. 

In this section the subject of multireactant enzymes will be introduced by examining some common bireactant systems. We will indicate the ligands as A and B, where B is a substrate and A can be a cosubstrate or coenzyme or an essential activator. 

In cellular metabolism, groups of enzymes work together in sequential pathways to carry out a given metabolic process, such as the multireaction breakdown of glucose to lactate or the multireaction synthesis of an amino acid from simpler precursors. In such enzyme systems, the reaction product of one enzyme becomes the substrate of the next. 

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